Browsing by Author "Cramb, David T."
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Item Open Access Application of fluorescence spectroscopy to the study of general anesthetic action at the molecular level(2003) Carnini, Anna; Cramb, David T.Item Open Access Cdse/zns core/shell quantum dot distribution in blood vessls of the chicken embryo chorioallantoic membrane(2007) Clancy, Aisling A.; Cramb, David T.Item Open Access Changes in asphaltene microenvironments evidenced by fluorescence solvatochromism(2001) Pietraru, Gabriella-Maria; Cramb, David T.Item Open Access Characterization of quanternary pyridinium surface films(2001) Wong, Jennifer E.; Cramb, David T.Item Open Access Comparing nanoparticle physical properties with bio-uptake(2012) Yaehne, Kristin Lee; Cramb, David T.Nanoparticle-organismal interactions depend on nanoparticle (NP) physical properties and tissue type. Angiogenic vasculature, characteristic of neoplastic diseases and developing tissues, has fenestrations between endothelial cells in which NPs can accumulate. This phenomenon is integral to nanomedicine and nanotoxicology, so we systemically injected dye-impregnated, polystyrene FluoSpheres (FS) into angiogenic chicken embryo chorioallantoic membrane (CAM) to determine bioaccumulation patterns and associated kinetics within the blood. FS concentration was monitored in the CAM using two-photon excitation fluorescence correlation spectroscopy and bio-uptake rate constants in flowing vessels were determined. Negatively charged, carboxylate-coated FS under 500 nm in diameter showed uptake in flowing vessels, while amine FS immediately agglomerated and adhered to the endothelium. Carboxylate FS uptake rate constants showed linear dependence on R-;/, indicating that NP surface area footprint determines uptake rates. A predictive bio-uptake model was developed based on NP physical properties, which clarifies the passive nature of NP uptake into angiogenic tissues.Item Open Access Item Open Access DNA double strand break repair: a biophysical study(2004) Merkle, Dennis; Cramb, David T.Item Open Access Fluid flow effects on nanoparticle localization in zebrafish vessels and cultured human endothelial cells(2017) Gomez, Maria Juliana; Rinker, Kristina D.; Childs, Sarah J.; Cramb, David T.; Di Martino, ElenaAssessment of nanoparticle distribution in the vasculature is important for determining drug delivery, molecular imaging efficacy, and risk profiles. Even though most medical nanoparticle applications require a vascular administration, factors affecting nanoparticle association with vessel walls in the presence of fluid forces are poorly understood. We evaluated the effect of fluid flow on the distribution of 200 nm carboxylate-coated polystyrene nanoparticles in flow-exposed endothelial cell cultures and zebrafish embryos. We combined confocal imaging of nanoparticle injected transgenic zebrafish, 3D modeling, and computational fluid dynamics to assess nanoparticle distribution under flow. Highest nanoparticle localization occurred in regions of disturbed flow and low shear stress found at branch points and downstream of bumps and curves in the zebrafish vasculature. Similar findings were obtained in human endothelial cells in vitro. Overall, fluid shear stress magnitude, flow disturbances, and flow-induced changes in endothelial physiology contribute to the vascular localization of nanoparticles.Item Open Access Function from structure: design, synthesis, and physiochemical characterization of fexible metal-sulfonate materials(2007) Chandler, Brett Donald; Shimizu, George K. H.; Cramb, David T.Item Open Access Investigations into two-photon photodynamic therapy(2002) Goyan, Rebecca Lynn; Cramb, David T.Item Open Access Nanoparticles for tolerance induction: analyzing key properties in solution and the chicken embryo chorioallantoic membrane(2011) Tekrony, Amy Danielle; Cramb, David T.Item Open Access Photokinetics and subcellular localizations of porphyrin sensitizers used in photodynamic therapy(2008) Kelly, Nicole M.; Cramb, David T.Item Open Access Quantum dot bioaccululation in angiogenic tissue: towards a physicochemical basis for nanotoxicity(2011) Gregoriou, Yiota; Cramb, David T.Item Open Access Two-photon excitation photodynamic therapy: progress towards a new treatment for wet age-related macular degeneration(2007) Samkoe, Kimberley Susanne; Cramb, David T.