Browsing by Author "Mitchell, Wendy G"

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    Pathogenic Cav3.2 channel mutation in a child with primary generalized epilepsy
    (2019-10-24) Souza, Ivana A; Gandini, Maria A; Zhang, Fang-Xiong; Mitchell, Wendy G; Matsumoto, Joyce; Lerner, Jason; Pierson, Tyler M; Zamponi, Gerald W
    Abstract Two paternally-inherited missense variants in CACNA1H were identified and characterized in a 6-year-old child with generalized epilepsy. Febrile and unprovoked seizures were present in this child. Both variants were expressed in cis or isolation using human recombinant Cav3.2 calcium channels in tsA-201 cells. Whole-cell patch-clamp recordings indicated that one variant (c.3844C > T; p.R1282W) caused a significant increase in current density consistent with a pathogenic gain-of-function phenotype; while the other cis-related variant (c.5294C > T; p.A1765V) had a benign profile.