Seafloor Sediment Bacterial Community Profiling for Baselines and Environmental Effects Monitoring at a Deep-Sea Oil Production Site Offshore Nova Scotia, Canada

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dc.contributor.advisorHubert, Casey R. J.
dc.contributor.authorStacey, Deidra Kathryn
dc.contributor.committeememberDunfield, Peter F.
dc.contributor.committeememberVamosi, Steven M.
dc.contributor.committeememberElse, Brent G. T.
dc.date2019-06
dc.date.accessioned2019-02-25T23:01:39Z
dc.date.available2019-02-25T23:01:39Z
dc.date.issued2019-02-22
dc.description.abstractMonitoring effects of environmental pollution is a critical aspect to preserving ecosystem health, but is challenging if baseline conditions are never established. Microorganisms are the first responders in a marine pollution event, hence oil-degrading bacteria can be used to monitor dispersion and biodegradation of oil spills. Deep-water subsurface oil reservoirs are predicted to exist along the Scotian Slope offshore Nova Scotia. Seafloor sediment from 19 Scotian Slope stations spanning a ~70,000 km2 area were used to generate 51 bacterial 16S rRNA gene amplicon libraries (V3-V4 region) to form a DNA baseline. A 300-day-long mock oil spill experiment using Scotian Slope sediment identified potential bacterial bioindicators of pristine and contaminated conditions, relative to baseline, underpinning an environmental monitoring approach that is proposed. This study shows that bacterial rRNA gene amplicon sequencing offers a novel parameter for baselines and environmentally responsible development of offshore deep-water oil drilling in Canada and beyond.en_US
dc.identifier.citationStacey, D. K. (2019). Seafloor Sediment Bacterial Community Profiling for Baselines and Environmental Effects Monitoring at a Deep-Sea Oil Production Site Offshore Nova Scotia, Canada (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/36150
dc.identifier.urihttp://hdl.handle.net/1880/109917
dc.language.isoenen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectEnvironmental Effects Monitoring, Environmental baselines, Microbial ecology, Nova Scotia, Marine pollution, Offshore oil, Marine environmental monitoring, Marine ecosystem monitoring, 16S rRNA geneen_US
dc.subject.classificationBioinformaticsen_US
dc.subject.classificationEcologyen_US
dc.subject.classificationMicrobiologyen_US
dc.subject.classificationBiology--Molecularen_US
dc.subject.classificationOceanographyen_US
dc.titleSeafloor Sediment Bacterial Community Profiling for Baselines and Environmental Effects Monitoring at a Deep-Sea Oil Production Site Offshore Nova Scotia, Canadaen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
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