The Immune Modulatory Effects of Tylvalosin in Porcine Neutrophils and Macrophages in vitro

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atmire.migration.oldid5747
dc.contributor.advisorBuret, Andre
dc.contributor.authorMoges, Ruth
dc.contributor.committeememberMorck, Douglas
dc.contributor.committeememberStorey, Douglas
dc.date.accessioned2017-07-11T15:27:50Z
dc.date.available2017-07-11T15:27:50Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractTylavlosin (TYL), a veterinary macrolide antibiotic, has been reported to have superior efficacy treating bacterial infections of the respiratory tract in swine. This study aims to characterize potential pro-resolving of tylvalosin benefits in a porcine model by elucidating its effects, in isolated neutrophils and monocyte-derived macrophages from piglets. Our findings indicate that TYL increases porcine neutrophil and macrophage apoptosis in a dose-dependent and time-dependent manners, without affecting levels of necrosis. TYL also modulates mediators of inflammation, by increasing pro-resolving lipid mediators (LXA4 and RvD1) in neutrophils and down regulating the pro- inflammatory mediators in stimulated neutrophils (LTB4) and in stimulated macrophages (CXCL8 and IL-1α). Together, these findings demonstrate that tylvalosin has immuno- modulating properties in vitro, including the induction of leukocyte apoptosis, the inhibition of pro-inflammatory cytokines and lipids mediators, and the induction of pro- resolving lipid mediators. Future studies using live piglets will help determine whether and how these effects may translate into anti-inflammatory benefits in the content of inflammatory disease within the lung.en_US
dc.identifier.citationMoges, R. (2017). The Immune Modulatory Effects of Tylvalosin in Porcine Neutrophils and Macrophages in vitro (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28175en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28175
dc.identifier.urihttp://hdl.handle.net/11023/3941
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subject.otherTylvalosin
dc.subject.otherMacrolide
dc.subject.otherAntibiotic
dc.subject.otherPorcine
dc.subject.otherNeutrophil
dc.subject.otherMacropahge
dc.subject.otherLipid Mediators
dc.subject.otherPro-Resolution
dc.titleThe Immune Modulatory Effects of Tylvalosin in Porcine Neutrophils and Macrophages in vitro
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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