Plxna4 and its role in neuronal survival in the developing zebrafish hindbrain

Simple item page
dc.contributor.advisorMcFarlane, Sarah
dc.contributor.authorNurcombe, Zachary W.
dc.contributor.committeememberKurrasch-Orbaugh, Deborah M.
dc.contributor.committeememberOusman, Shalina S.
dc.contributor.committeememberMains, Paul E.
dc.date2019-11
dc.date.accessioned2019-09-18T20:41:35Z
dc.date.available2019-09-18T20:41:35Z
dc.date.issued2019-09-17
dc.description.abstractPlexins are a family of transmembrane proteins that bind Semaphorin ligands, and are known to function in axon guidance. In embryonic zebrafish, I find that plxna4 is expressed widely in the nervous system early in development, and localizes to the hindbrain as neurogenesis and differentiation proceed. In the hindbrain of a plxna4-/- CRISPR mutant, expression of the Islet transcription factor by cranial motor neurons appears depleted at 48 hours post fertilization, with increased apoptosis at 24 hpf. These data support a survival role for Plexina4 in the embryonic zebrafish hindbrain. Literature indicates that the secreted heat shock protein Clusterin is a candidate ligand to mediate apoptosis through Plexina4, as Clusterin is known to promote cell survival, and bind Plxna4. I find that clu is expressed by the floorplate of the embryonic zebrafish hindbrain. Epistasis experiments reveal Clusterin and Plexina4 promote cell survival in the developing zebrafish hindbrain via independent pathways.en_US
dc.identifier.citationNurcombe, Z. W. (2019). Plxna4 and its role in neuronal survival in the developing zebrafish hindbrain (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37071
dc.identifier.urihttp://hdl.handle.net/1880/111006
dc.language.isoengen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectcell survivalen_US
dc.subjectdevelopmenten_US
dc.subjectneuroscienceen_US
dc.subjectPlexinen_US
dc.subjectzebrafishen_US
dc.subjecthindbrainen_US
dc.subject.classificationBiology--Cellen_US
dc.subject.classificationBiology--Molecularen_US
dc.subject.classificationNeuroscienceen_US
dc.titlePlxna4 and its role in neuronal survival in the developing zebrafish hindbrainen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Neuroscienceen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ucalgary_2019_nurcombe_zachary.pdf
Size:
75.67 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Open Theses and Dissertations