Surface interactions and cartilage damage in two ovine models of stifle injury

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dc.contributor.advisorFrank, Cyril B.
dc.contributor.authorBeveridge, Jillian Elizabeth
dc.date.accessioned2017-12-18T22:31:15Z
dc.date.available2017-12-18T22:31:15Z
dc.date.issued2012
dc.descriptionBibliography: p. 251-285en
dc.descriptionSome pages are in colour.en
dc.descriptionIncludes copies of animal protocol approvals. Original copies with original Partial Copyright Licence.en
dc.description.abstractAnterior cruciate ligament and meniscus tears are injuries that are known risk factors for post-traumatic OA in humans, presumably because of residual abnormal knee mechanics. This work sought to determine whether abnormal motion of the tibiofemoral joint surfaces was related to early cartilage damage in two ovine joint injury models: combined anterior cruciate and medial collateral ligament transection (ACL/MCLx), and lateral meniscectomy (Mx). Five descriptions of in vivo tibiofemoral inter-surface motion (i.e., "surface interactions") were selected based on their speculated relationship to mechanical mechanisms known to damage cartilage in vitro. The hypotheses were: H1. increasingly abnormal surface interactions would correlate with cartilage damage, H2. the number of significant correlations would be greatest in ACL/MCLx sheep, and H3. locations of cartilage damage would qualitatively coincide with. that of altered surface interactions. 21 sheep were allocated to four groups: 6 ACL/MCLx, 5 Mx, 4 sham-operated, and 6 non-operated controls. Baseline in vivo kinematics were measured prior to injury, and serially up to 20 weeks post­injury, and expressed as the difference from Intact. Cartilage damage was quantified and mapped at sacrifice. Repeated measures ANOVAs and Pearson correlation coefficients were used to determine whether surface interactions were significantly different from Intact and correlated with cartilage damage. Results partially supported the hypotheses. ACL/MCLx surface interactions were significantly abnormal, and 3/5 interactions correlated with the magnitude of cartilage damage but not its distribution. The number of significant correlations was greatest in ACL/MCLx sheep and had a cumulative effect on cartilage damage that was individual- and surface-specific, suggesting that different surface interactions may be more damaging in different individuals. Lateral compartment joint space was significantly reduced following meniscectomy, but only correlated with cartilage damage at discrete gait points for two surface interactions 20 weeks post-Mx, suggesting that surface interactions presently quantified did not reflect the primary mechanism responsible for damage in this model, likely contact stress. The presence of significant correlations between multiple surface interactions and cartilage damage in both injury models add strong support to the paradigm that altered mechanics play a pivotal role in the aetiology of early cartilage damage following these joint injuries.
dc.format.extentxxix, 294 leaves : ill. ; 30 cm.en
dc.identifier.citationBeveridge, J. E. (2012). Surface interactions and cartilage damage in two ovine models of stifle injury (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4749en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4749
dc.identifier.urihttp://hdl.handle.net/1880/105750
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleSurface interactions and cartilage damage in two ovine models of stifle injury
dc.typedoctoral thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2082 627942954
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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