Conservation genomics of the endangered Banff Springs Snail (Physella johnsoni) using Pool-seq
| dc.contributor.advisor | Rogers, Sean | |
| dc.contributor.author | Stanford, Brenna | |
| dc.contributor.committeemember | Lepitzki, Dwayne | |
| dc.contributor.committeemember | Vamosi, Jana | |
| dc.date | 2019-06 | |
| dc.date.accessioned | 2019-01-14T17:22:18Z | |
| dc.date.available | 2019-01-14T17:22:18Z | |
| dc.date.issued | 2019-01-07 | |
| dc.description.abstract | Understanding how species persist and adapt to local habitats is a fundamental question for species of conservation concern. Located in Banff National Park, the endangered snail, Physella johnsoni, inhabits seven highly specialized thermal springs. P. johnsoni undergo yearly population bottlenecks with minimal to no dispersal among springs. The consequences of these processes on genetic population structure are unknown. To investigate effects of habitat and life history on P. johnsoni’s genome and to test the hypothesis of a single panmictic population, I collected 20 to 40 snails/population for P. johnsoni and a closely related snail, P. gyrina, in adjacent, non-thermal water. Using whole genome pooled-sequencing, millions of single nucleotide polymorphisms were captured. These genetic variants resolved significant genetic divergence between P. johnsoni and P. gyrina. In addition, I detected distinct genetic clusters and reduced nucleotide diversity within each spring, indicative of strong micro-geographical population structure and suggestive of a role for genetic drift. These results suggest that P. johnsoni from each spring represent a distinct genetic unit, which has conservation implications for the designation of designatable unit status under COSEWIC, and where mixing of snails may reduce the consequences of genetic drift. | en_US |
| dc.identifier.citation | Stanford, B. (2019). Conservation genomics of the endangered Banff Springs Snail (Physella johnsoni) using Pool-seq (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/35709 | |
| dc.identifier.uri | http://hdl.handle.net/1880/109445 | |
| dc.language.iso | en | en_US |
| dc.publisher.faculty | Science | en_US |
| dc.publisher.institution | University of Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | en_US |
| dc.subject.classification | Education--Sciences | en_US |
| dc.subject.classification | Bioinformatics | en_US |
| dc.subject.classification | Ecology | en_US |
| dc.subject.classification | Genetics | en_US |
| dc.subject.classification | Biology--Molecular | en_US |
| dc.title | Conservation genomics of the endangered Banff Springs Snail (Physella johnsoni) using Pool-seq | en_US |
| dc.type | master thesis | en_US |
| thesis.degree.discipline | Biological Sciences | en_US |
| thesis.degree.grantor | University of Calgary | en_US |
| thesis.degree.name | Master of Science (MSc) | en_US |
| ucalgary.item.requestcopy | true |