Transcriptome Profiling in Ephedra sinica and Catha edulis Reveals Enzymes Putatively Involved in Ephedrine Alkaloid Biosynthesis

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atmire.migration.oldid4014
dc.contributor.advisorFacchini, Peter
dc.contributor.authorGroves, Ryan A
dc.contributor.committeememberMoorhead, Gregory
dc.contributor.committeememberRo, Dae-Kyun
dc.contributor.committeememberStrous, Marc
dc.contributor.committeememberVogel, Hans
dc.date.accessioned2016-01-14T20:45:01Z
dc.date.available2016-01-14T20:45:01Z
dc.date.issued2016-01-14
dc.date.submitted2016en
dc.description.abstractAmphetamine analogs are a class of medicinal compounds produced by Ephedra sinica and Catha edulis. Two commonly used members of this class of compounds include the cough suppressant (1S-2S)-pseudoephedrine, and the stimulant (1R-2S)-ephedrine. These secondary metabolites are produced in planta through a L-phenylalanine derived multi-step pathway. Despite the importance of this biosynthetic pathway, only one enzyme in the pathway has been characterized at the molecular level. Recently, two new transcriptomes have been created for E. sinica and C. edulis with the hopes of facilitating future gene discovery in this untapped pathway. In this investigation, I outlined an efficient transcriptomics based approach to potential gene candidate selection for all putative stages of the amphetamine analog biosynthetic pathway. This investigation also describes a complete approach to molecular cloning, protein expression, and assaying of gene candidates, resulting in the discovery of a key biosynthetic enzyme involved in amphetamine analog biosynthesis in E. sinica.en_US
dc.identifier.citationGroves, R. A. (2016). Transcriptome Profiling in Ephedra sinica and Catha edulis Reveals Enzymes Putatively Involved in Ephedrine Alkaloid Biosynthesis (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28507en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28507
dc.identifier.urihttp://hdl.handle.net/11023/2747
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBioinformatics
dc.subjectGenetics
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subjectBiochemistry
dc.subjectChemistry--Pharmaceutical
dc.subject.classificationEphedraen_US
dc.subject.classificationKhaten_US
dc.subject.classificationAlkaloidsen_US
dc.subject.classificationBiosynthesisen_US
dc.subject.classificationDatabaseen_US
dc.subject.classificationEnzymesen_US
dc.subject.classificationRNA sequencingen_US
dc.subject.classificationBenzaldehydesen_US
dc.subject.classificationEphedrineen_US
dc.subject.classificationAmphetamine-analogueen_US
dc.subject.classificationTranscriptomeen_US
dc.titleTranscriptome Profiling in Ephedra sinica and Catha edulis Reveals Enzymes Putatively Involved in Ephedrine Alkaloid Biosynthesis
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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