Engineering of Gelatin Methacryloyl (GelMA) Hydrogels for Developing Biomimetic Tissue Constructs

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dc.contributor.advisorSanati Nezhad, Amir
dc.contributor.authorJanmaleki, Mohsen
dc.contributor.committeememberUngrin, Mark D.
dc.contributor.committeememberYipp, Bryan G.
dc.contributor.committeememberRonsky, Janet L.
dc.contributor.committeememberGaharwar, Akhilesh K.
dc.contributor.committeememberKhoshnazar, Rahil
dc.contributor.committeememberLu, Qingye G.
dc.dateWinter Conferral
dc.date.accessioned2022-11-15T17:43:12Z
dc.date.embargolift2022-10-14
dc.date.issued2020-10-14
dc.description.abstractThree-dimensional (3D) cell culture offers a more physiologically-relevant context for disease modeling and drug screening. Hydrogel-based biomaterials support the long-term culture of cells in 3D and enhance memetic cell-cell and cell-matrix interactions. Herein, gelatin methacryloyl (GelMA) hydrogel, a well-known photo-crosslinkable hydrogel, was selected for engineering of different tissue constructs.First, the practicability of imprinting cell topography on GelMA hydrogel was investigated. A novel method was developed to fabricate cell-like niches over the hydrogel’s substrate, and its effects on cytocompatibility and drug susceptibility of breast cancer cells were studied. Second, GelMA hydrogel was tuned in terms of mechanical properties and porosity to facilitate in vitro myelination of dorsal root ganglia (DRG) neurons by Schwann cells (SCs). It was shown that the tuned GelMA enhanced single axonal generation (unlike collagen) and promoted DRGs’ interaction with SCs (unlike PDL). Third, the role of temperature on bioprintability of GelMA bioinks in a two-step crosslinking strategy was investigated. Lowering the temperature can enhance the physical gelation of GelMA and consequently improve filament formation. Results showed that the decrease in the temperature could improve the printability and shape fidelity of the deposited hydrogel, particularly at 15 °C. Time-dependent mechanical testing confirmed higher elastic properties of the collected hydrogel at the lower temperature.Fourth, a hydrogel-based 3D human intracranial aneurysm (IA) model was developed using liquid assisted injection molding. With clinically relevant dimensions and tuned fluidic and matrix properties, the essential endothelium was successfully lined inside the reconstructed IA over pre-cultured smooth muscle cells. Based on the characterized viscoelastic properties of the GelMA hydrogel and with the help of a fluid-structure interaction model, the capability of the IA construct model in predicting the response of the IA to different fluid flow profiles was demonstrated.Finally, using the techniques developed in this thesis, a new approach is suggested to fabricate a fully hydrogel-based platform for tissue engineering and organ-a-chip applications.
dc.identifier.citationJanmaleki, M. (2020). Engineering of Gelatin Methacryloyl (GelMA) Hydrogels for Developing Biomimetic Tissue Constructs (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.urihttp://hdl.handle.net/1880/115482
dc.identifier.urihttps://dx.doi.org/10.11575/PRISM/40449
dc.language.isoenen
dc.language.isoEnglish
dc.publisher.facultyGraduate Studiesen
dc.publisher.facultySchulich School of Engineering
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en
dc.subjectHydrogel
dc.subjectOrgan-on-a-chip
dc.subjectImprinted substrates
dc.subjectMechanical behavior
dc.subjectMyelination
dc.subjectschwann cells
dc.subjectBioprinter
dc.subjectThermoreversible crosslinking
dc.subjectGelMA
dc.subjectFidelity
dc.subjectAneurysm
dc.subjectFluid-structure interaction
dc.subjectHydrogel-based organ-on-chip
dc.subject.classificationBiology--Cell
dc.subject.classificationBiology--Neuroscience
dc.subject.classificationEngineering--Biomedical
dc.subject.classificationEngineering--Materials Science
dc.subject.classificationEngineering--Mechanical
dc.titleEngineering of Gelatin Methacryloyl (GelMA) Hydrogels for Developing Biomimetic Tissue Constructs
dc.typedoctoral thesis
thesis.degree.disciplineEngineering – Biomedical
thesis.degree.grantorUniversity of Calgaryen
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
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