Methane oxidation and methylotroph population dynamics in groundwater mesocosms

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dc.contributor.authorKuloyo, Olukayode
dc.contributor.authorRuff, S Emil
dc.contributor.authorCahill, Aaron
dc.contributor.authorConnors, Liam
dc.contributor.authorZorz, Jackie K
dc.contributor.authorHrabe de Angelis, Isabella
dc.contributor.authorNightingale, Michael
dc.contributor.authorMayer, Bernhard
dc.contributor.authorStrous, Marc
dc.date.accessioned2022-09-02T22:33:57Z
dc.date.available2022-09-02T22:33:57Z
dc.date.issued2020-01
dc.description.abstractExtraction of natural gas from unconventional hydrocarbon reservoirs by hydraulic fracturing raises concerns about methane migration into groundwater. Microbial methane oxidation can be a significant methane sink. Here, we inoculated replicated, sand-packed, continuous mesocosms with groundwater from a field methane release experiment. The mesocosms experienced thirty-five weeks of dynamic methane, oxygen and nitrate concentrations. We determined concentrations and stable isotope signatures of methane, carbon dioxide and nitrate and monitored microbial community composition of suspended and attached biomass. Methane oxidation was strictly dependent on oxygen availability and led to enrichment of 13 C in residual methane. Nitrate did not enhance methane oxidation under oxygen limitation. Methylotrophs persisted for weeks in the absence of methane, making them a powerful marker for active as well as past methane leaks. Thirty-nine distinct populations of methylotrophic bacteria were observed. Methylotrophs mainly occurred attached to sediment particles. Abundances of methanotrophs and other methylotrophs were roughly similar across all samples, pointing at transfer of metabolites from the former to the latter. Two populations of Gracilibacteria (Candidate Phyla Radiation) displayed successive blooms, potentially triggered by a period of methane famine. This study will guide interpretation of future field studies and provides increased understanding of methylotroph ecophysiology.en_US
dc.description.grantingagencyNatural Sciences and Engineering Research Council (NSERC)en_US
dc.description.grantingagencyAlberta Innovates - Research Granten_US
dc.description.grantingagencyUniversity of Calgary - Research Granten_US
dc.description.grantingagencyOtheren_US
dc.identifier.citationKuloyo, O., Ruff, S. E., Cahill, A., Connors, L., Zorz, J. K., Hrabe de Angelis, I., Nightingale, M., Mayer, B., & Strous, M. (2020). Methane oxidation and methylotroph population dynamics in groundwater mesocosms. Environmental Microbiology, 22(4), 1222–1237. https://doi.org/10.1111/1462-2920.14929en_US
dc.identifier.doihttp://dx.doi.org/10.1111/1462-2920.14929en_US
dc.identifier.grantnumberGrant no. 463045-14en_US
dc.identifier.urihttp://hdl.handle.net/1880/115164
dc.identifier.urihttps://doi.org/10.11575/PRISM/46077
dc.language.isoengen_US
dc.publisherSociety for Applied Microbiology and John Wiley & Sons Ltd.en_US
dc.publisher.departmentGeoscienceen_US
dc.publisher.facultyScienceen_US
dc.publisher.hasversionpublishedVersionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.14929en_US
dc.rightsUnless otherwise indicated, this material is protected by copyright and has been made available with authorization from the copyright owner. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleMethane oxidation and methylotroph population dynamics in groundwater mesocosmsen_US
ucalgary.item.requestcopyfalseen_US
ucalgary.scholar.levelFacultyen_US
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