Characterizing Inhibitor of Growth (ING) family evolution and ING1 structure and function

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dc.contributor.advisorRiabowol, Karl T.
dc.contributor.authorBertschmann, Jessica
dc.contributor.committeememberCobb, Jennifer A.
dc.contributor.committeememberDe Koning, A. P. Jason
dc.date2020-11
dc.date.accessioned2020-07-31T20:45:15Z
dc.date.available2020-07-31T20:45:15Z
dc.date.issued2020-07-29
dc.description.abstractThe INhibitior of Growth (ING) family of tumor suppressors have emerged as a versatile family of phospholipid effectors, histone mark sensors, and growth regulators. An updated phylogenetic analysis of this protein family using sequences from 42 eukaryotic species reveals that ING4 is likely most similar to the ancestral ING protein, not ING3 as previously reported. Previous studies have shown that the major ING1 isoforms, ING1a and ING1b serve distinct cellular functions by differentially regulating apoptosis and senescence in primary cells. The ING1a isoform encodes a sequence unique in the human proteome. To identify ING1a homologs in other species we searched all available databases and found that sequences corresponding to ING1a were only found in great apes and Old-World monkeys. However, only select primates had start codons capable of encoding full-length ING1a. Moreover, when we expressed ING1a with and without it’s unique N-terminal sequence, the unique sequence promoted localization to the mitochondria. Given the natural induction of this isoform as cells age in culture, expression of ING1a may serve to help limit the replicative lifespan of cells from long-lived primates, in part through its activity in the mitochondria.en_US
dc.identifier.citationBertschmann, J. (2020). Characterizing Inhibitor of Growth (ING) family evolution and ING1 structure and function (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/38058
dc.identifier.urihttp://hdl.handle.net/1880/112355
dc.language.isoengen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectEvolutionen_US
dc.subjectMitochondriaen_US
dc.subjectEpigeneticsen_US
dc.subject.classificationBiology--Molecularen_US
dc.titleCharacterizing Inhibitor of Growth (ING) family evolution and ING1 structure and functionen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Biochemistry and Molecular Biologyen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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