Nitric Oxide Mediated Antiviral Response in Avian Cells

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atmire.migration.oldid1582
dc.contributor.advisorAbdul-Careem, Mohamed Faizal
dc.contributor.authorHaddadi, Siamak
dc.date.accessioned2013-10-02T20:24:55Z
dc.date.available2013-11-12T08:00:19Z
dc.date.issued2013-10-02
dc.date.submitted2013en
dc.description.abstractLPS activates TLR4 signaling pathway eliciting antiviral host responses in mammals although information on such responses in avian species is scarce. Objectives of the work described in the thesis were to 1. characterize the LPS induced expression of LPS receptors and NO in two avian cell lines, LMH and MQ-NCSU, and 2. observe whether NO can elicit antiviral response against ILTV replication. We found that LPS was capable of inducing the expression of TLR4, CD14 and NO production only in MQ-NCSU cells. We also showed that TLR4 mediated NO production in MQ-NCSU cells confers antiviral response against ILTV in susceptible LMH cells. Using a selective inhibitor of inducible NO synthase and a NO donor as a source of NO, we confirmed that this effect is positively correlated with NO. Our data showed that LPS can be a potential innate immune stimulant that can be used against ILTV infection in chickens.en_US
dc.identifier.citationHaddadi, S. (2013). Nitric Oxide Mediated Antiviral Response in Avian Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27106en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27106
dc.identifier.urihttp://hdl.handle.net/11023/1086
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectVeterinary Science
dc.subjectVirology
dc.subjectImmunology
dc.subject.classificationinfectious laryngotracheitis virusen_US
dc.subject.classificationlipopolysaccharideen_US
dc.subject.classificationnitric oxideen_US
dc.subject.classificationTLR4en_US
dc.titleNitric Oxide Mediated Antiviral Response in Avian Cells
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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