Investigating T Cell Exhaustion in Multiple Myeloma

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dc.contributor.advisorBahlis, Nizar
dc.contributor.authorBarakat, Elie
dc.contributor.committeememberMahoney, Douglas
dc.contributor.committeememberYong, Wee
dc.date2023-06
dc.date.accessioned2023-04-27T19:38:04Z
dc.date.available2023-04-27T19:38:04Z
dc.date.issued2023-04-26
dc.description.abstractMultiple Myeloma (MM) is a currently incurable malignancy of plasma cells. Immunotherapies are widely agreed upon to be the most likely avenue through which a cure could be found, however, to become longer lasting, permanent solutions, immunotherapies must overcome persistent antigenic stimulation and immune exhaustion. To this end, there is no currently agreed upon immune signature that denotes which cells are exhausted, and which are healthy. The aim of this thesis was to define this exhaustion signature, using only surface antigens and flow cytometry, in order to sort these exhausted T cells from peripheral blood mononuclear cells (PBMC). Based upon the logic that patients who respond poorly to immunotherapy will have on average more exhausted T cells than patients who respond well to immunotherapy, this project developed and optimized a flow cytometry panel designed to use 14 different T cell markers to phenotypically characterize T cells isolated from patient bone marrow aspirates (BMA). While this project did not succeed in generating a full exhaustion signature due to issues concerning censorship within the cohort which shrunk the sample size, it does show that a repetition of this experiment could elucidate the exhaustion signature with a larger sample size and slight adjustments to the antibody panel. An exhaustion signature for T cells can be generated, using surface markers that can be used for flow cytometric sorting in the future.
dc.identifier.citationBarakat, E. (2023). Investigating T cell exhaustion in multiple myeloma (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.urihttp://hdl.handle.net/1880/116123
dc.identifier.urihttps://dx.doi.org/10.11575/PRISM/dspace/40968
dc.language.isoen
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgary
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject.classificationOncology
dc.titleInvestigating T Cell Exhaustion in Multiple Myeloma
dc.typemaster thesis
thesis.degree.disciplineMedicine – Medical Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.thesis.accesssetbystudentI do not require a thesis withhold – my thesis will have open access and can be viewed and downloaded publicly as soon as possible.
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