Activation of latent Escherichia coli virulence factors by Campylobacter jejuni

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atmire.migration.oldid2274
dc.contributor.advisorBuret, Andre
dc.contributor.authorReti, Kristen
dc.date.accessioned2014-07-11T17:05:45Z
dc.date.embargolift2016-07-10T17:05:45Z
dc.date.issued2014-07-11
dc.date.submitted2014en
dc.description.abstractCampylobacter jejuni is the most common cause of bacterial gastroenteritis, and while typically self-limiting, C. jejuni infections are associated with post-infectious intestinal disorders, including inflammatory bowel disease and irritable bowel syndrome. This study sought to determine if C. jejuni induces virulence in commensal, non-invasive E. coli. Expression of adhesin, flagella, hemolysin, biofilm formation, and antibiotic resistance genes were increased in E. coli upon exposure to C. jejuni-conditioned media. Atomic force microscopy demonstrated E. coli was more adherent to human colonic epithelial cells when exposed to C. jejuni-conditioned media. C. jejuni and C. jejuni-conditioned media also induced E. coli flagella expression. In vitro, this altered E. coli phenotype disrupted TLR4 expression and induced IL-8 secretion. These data suggest C. jejuni and C. jejuni-conditioned media can induce virulence in non-invasive, commensal E. coli, and this contributes to host inflammation. These results provide a novel mechanism for post-infectious complications.en_US
dc.description.embargoterms2 yearsen_US
dc.identifier.citationReti, K. (2014). Activation of latent Escherichia coli virulence factors by Campylobacter jejuni (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24690en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/24690
dc.identifier.urihttp://hdl.handle.net/11023/1622
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subject.classificationCampylobacteren_US
dc.subject.classificationPost-infectiousen_US
dc.subject.classificationInflammatory Bowel Diseaseen_US
dc.subject.classificationIrritable Bowel Syndromeen_US
dc.titleActivation of latent Escherichia coli virulence factors by Campylobacter jejuni
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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