The Role of Prolactin Receptors in Regulation of Pancreatic Beta Cell Mass and Function

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atmire.migration.oldid4471
dc.contributor.advisorHuang, Carol
dc.contributor.authorShrivastava, Vipul
dc.contributor.committeememberCross, James
dc.contributor.committeememberSlater, Donna
dc.date.accessioned2016-05-20T14:47:10Z
dc.date.available2016-05-20T14:47:10Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractDuring pregnancy, pancreatic β-cells adapt to the increase in maternal insulin resistance by up-regulating β-cell mass, insulin synthesis, and lowering glucose-stimulated insulin secretion threshold. Signaling through prolactin receptor (PrlR) is critical for these adaptive responses. We hypothesize that PrlR present on β-cells are the primary determinant of β-cell adaptation to pregnancy. We found that β-cell specific deletion of PrlR in mice leads to higher fasted blood glucose and impaired glucose tolerance in comparison with wild type mice. Furthermore, we identified Lrrc55 (leucine rich repeat containing 55), an auxiliary subunit of BK channels as one of the potentially novel targets of PrlR. Results suggest that Lrrc55 overexpression protected INS-1 cells from H2O2, high glucose, palmitate, and high glucose+palmitate-induced apoptosis by attenuating ER stress and intrinsic apoptosis pathways and also maintaining healthy ER calcium reserves. Taken together, this study helps unravel components of PrlR signaling as possible therapeutic strategy to treat diabetes.en_US
dc.identifier.citationShrivastava, V. (2016). The Role of Prolactin Receptors in Regulation of Pancreatic Beta Cell Mass and Function (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28348en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28348
dc.identifier.urihttp://hdl.handle.net/11023/3032
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyMedicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology
dc.subjectBioinformatics
dc.subjectBiostatistics
dc.subjectBiology--Cell
dc.subjectGenetics
dc.subject.classificationDiabetesen_US
dc.titleThe Role of Prolactin Receptors in Regulation of Pancreatic Beta Cell Mass and Function
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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