Epitope Mapping of Bovine Viral Diarrhea Virus Antigens E1, E2, Erns, and NS3 using Phage Display and Peptide Scanning

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dc.contributor.advisorvan der Meer, Frank
dc.contributor.authorBremner, William T. R.
dc.contributor.committeememberSchryvers, Anthony Bernard
dc.contributor.committeememberCoffin, Carla S.
dc.contributor.committeemembervan Marle, Guido
dc.date2021-02
dc.date.accessioned2021-01-06T21:55:48Z
dc.date.available2021-01-06T21:55:48Z
dc.date.issued2020-12-21
dc.description.abstractBovine viral diarrhea virus is a cattle pathogen with global distribution, and substantial economic impact. Control of viral transmission is challenging by the lifelong viral shedding by persistently infected (PI) animals, and the significant diversity of the virus. While surveillance and removal of PI animals is the primary focus of control programs, insight into the antigenicity of the virus could be valuable for developing broadly protective vaccines to reduce the spread of the virus within farms and in this way the incidence of persistently infected animals (PI’s). This study aims to characterise the viral proteins of BVDV which elicit specific antibody responses. These proteins are the surface glycoproteins E1, E2 and Erns, as well as the non-structural protein NS3. This work employs the techniques of phage display, and peptide scanning to identify epitope structures on these four antigens and puts them into the context of existing structural models. Linear epitopes of potential interest for future vaccine development were identified on each of the four antigens. While attempts at characterizing conformational epitopes highlighted shortcomings in the computational workflow for such efforts, valuable insight into the limitations and future directions are drawn.en_US
dc.identifier.citationBremner, W. T. R. (2020). Epitope Mapping of Bovine Viral Diarrhea Virus Antigens E1, E2, Erns, and NS3 using Phage Display and Peptide Scanning (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/38529
dc.identifier.urihttp://hdl.handle.net/1880/112937
dc.language.isoengen_US
dc.publisher.facultyVeterinary Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectBVDVen_US
dc.subjectAntibodyen_US
dc.subjectVirologyen_US
dc.subject.classificationMicrobiologyen_US
dc.subject.classificationImmunologyen_US
dc.titleEpitope Mapping of Bovine Viral Diarrhea Virus Antigens E1, E2, Erns, and NS3 using Phage Display and Peptide Scanningen_US
dc.typemaster thesisen_US
thesis.degree.disciplineVeterinary Medical Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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