Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou

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atmire.migration.oldid4246
dc.contributor.advisorGilch, Sabine
dc.contributor.authorCheng, Yo Ching
dc.contributor.committeememberJirik, Frank Robert
dc.contributor.committeememberKutz, Susan J.
dc.date.accessioned2016-04-21T18:31:45Z
dc.date.available2016-04-21T18:31:45Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractChronic wasting disease (CWD) is a transmissible prion disease affecting cervids mainly in North America. The responsible agent for this disease is the protease-resistant and infectious prion protein isoform (PrPSc) which is converted from a normal host-encoded glycoprotein (PrPC). In this study, I employed a novel in vitro amplification assay; the real-time quaking-induced conversion assay (RT-QuIC) to detect CWD prions in feces. I demonstrated the use of optimized RT-QuIC to define the shedding pattern of CWD prions in feces during the course of CWD. Additionally, I analyzed the distribution of PrP polymorphism among caribou populations in Alberta. The results permit predictions on the probability of CWD transmission to caribou populations. My studies provide a new tool to improve CWD surveillance and transmission risk assessment, and may be useful for CWD management in the future.en_US
dc.identifier.citationCheng, Y. C. (2016). Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25326en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25326
dc.identifier.urihttp://hdl.handle.net/11023/2896
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectAnimal Pathology
dc.subjectGenetics
dc.subjectBiology--Molecular
dc.subjectVeterinary Science
dc.subject.classificationChronic Wasting Diseaseen_US
dc.subject.classificationPrionsen_US
dc.subject.classificationPrion diseasesen_US
dc.subject.classificationdiagnosisen_US
dc.subject.classificationCaribouen_US
dc.subject.classificationElken_US
dc.subject.classificationSurveillanceen_US
dc.subject.classificationRT-QuICen_US
dc.titleChronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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