A Borrelia burgdorferi mini-vls system that undergoes antigenic switching in mice: investigation of the role of plasmid topology and the long inverted repeat

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dc.contributor.authorCastellanos, Mildred
dc.contributor.authorVerhey, Theodore B
dc.contributor.authorChaconas, George
dc.date.accessioned2018-12-07T23:16:44Z
dc.date.available2018-12-07T23:16:44Z
dc.date.issued2018-09
dc.description.abstractBorrelia burgdorferi evades the host immune system by switching the surface antigen. VlsE, in a process known as antigenic variation. The DNA mechanisms and genetic elements present on the vls locus that participate in the switching process remain to be elucidated. Manipulating the vls locus has been difficult due to its instability on Escherichia coli plasmids. In this study, we generated for the first time a mini-vls system composed of a single silent vlsE variable region (silent cassette 2) through the vlsE gene by performing some cloning steps directly in a highly transformable B. burgdorferi strain. Variants of the mini system were constructed with or without the long inverted repeat (IR) located upstream of vlsE and on both circular and linear plasmids to investigate the importance of the IR and plasmid topology on recombinational switching at vlsE. Amplicon sequencing using PacBio long read technology and analysis of the data with our recently reported pipeline and VAST software showed that the system undergoes switching in mice in both linear and circular versions and that the presence of the hairpin does not seem to be crucial in the linear version, however it is required when the topology is circular.en_US
dc.identifier.citationCastellanos, M., Verhey, T. B., & Chaconas, G. (2018). A Borrelia burgdorferi mini‐vls system that undergoes antigenic switching in mice: investigation of the role of plasmid topology and the long inverted repeat. Molecular microbiology, 109(5), 710-721.en_US
dc.identifier.doihttp://dx.doi.org/10.1111/mmi.14071en_US
dc.identifier.other29995993
dc.identifier.urihttp://hdl.handle.net/1880/109291
dc.identifier.urihttps://doi.org/10.11575/PRISM/43785
dc.language.isoenen_US
dc.publisherWileryen_US
dc.publisher.departmentBiochemistry & Molecular Biologyen_US
dc.publisher.departmentMicrobiology, Immunology & Infectious Diseasesen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.hasversionPost-printen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.htmlen_US
dc.subjectantigenic variationen_US
dc.subjectBorrelia burgdorferien_US
dc.subjectLyme diseaseen_US
dc.subjectvlsEen_US
dc.titleA Borrelia burgdorferi mini-vls system that undergoes antigenic switching in mice: investigation of the role of plasmid topology and the long inverted repeaten_US
dc.typejournal articleen_US
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