The Effect of Low Oxygen Level on Human Mesenchymal Stem Cell Behaviour in Culture

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atmire.migration.oldid4349
dc.contributor.advisorSen, Arindom
dc.contributor.authorHeik, Evelyn
dc.contributor.committeememberDuncan, Neil Alexander
dc.contributor.committeememberHart, David Arthur
dc.contributor.committeememberMurari, Kartikeya
dc.contributor.committeememberPonnurangam, Sathish
dc.date.accessioned2016-05-05T15:54:39Z
dc.date.available2016-05-05T15:54:39Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractSynovial fluid from articulating joints contain a population of mesenchymal stem cells (SF-MSCs), which have the ability to effectively form cartilage, and thus represent a potential candidate cell type for the development of therapies aimed at repairing cartilage lesions. Since they can only be isolated in small quantities and any treatment developed will require a large number of them, SF-MSCs for clinical use will have to be generated in culture under conditions that promote their rapid proliferation. Determining the optimal oxygen levels in serum-free medium, free of ill-defined components and infectious agents, would allow for a better understanding of SF-MSCs and their potential for use in clinical treatments. The findings of this study indicate that culturing SF-MSCs under serum-free conditions in static culture is feasible, and that low oxygen conditions may improve cell yield. Furthermore, the cells maintained their defining stem cell characteristics when cultured under low oxygen tension.en_US
dc.identifier.citationHeik, E. (2016). The Effect of Low Oxygen Level on Human Mesenchymal Stem Cell Behaviour in Culture (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28085en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28085
dc.identifier.urihttp://hdl.handle.net/11023/2975
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectEngineering--Biomedical
dc.subject.classificationmesenchymal stem cellsen_US
dc.subject.classificationSynovial Fluiden_US
dc.subject.classificationserum-free mediumen_US
dc.subject.classificationLow oxygenen_US
dc.titleThe Effect of Low Oxygen Level on Human Mesenchymal Stem Cell Behaviour in Culture
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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