Regulation of Cell Death by The Drosophila ING Proteins

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atmire.migration.oldid3220
dc.contributor.advisorRiabowol, Karl
dc.contributor.advisorGrewal, Savraj
dc.contributor.authorMobahat, Mahsa
dc.date.accessioned2015-05-04T15:59:07Z
dc.date.available2015-06-22T07:00:51Z
dc.date.issued2015-05-04
dc.date.submitted2015en
dc.description.abstractThe inhibitor of growth (ING) family of type II tumor suppressors (ING1-ING5) are involved in various cellular processes including apoptosis, DNA repair and tumor growth. INGs are subunits of histone deacetylase (HDAC) and histone acetyltransferase (HAT) complexes. The Drosophila genome encodes 3 ING homologues, dING 2, 3 and 4. In this research, I showed that overexpression of dING2 leads to smaller tissue and clone size in Drosophila. dING2 was sufficient to induce caspase-dependent but p53-independent cell death, which promoted expression of the pro-apoptotic gene, reaper. Experiments conducted on polyploid tissues revealed that clonal overexpression of dING2 had little effect in cells in the larval fat body, but resulted in smaller salivary glands. My experiments established that overexpression of dING3 induces caspase-dependent cell death. However, neither dING2 nor dING3 is required for IR-induced apoptosis. This work should provide valuable insights into the role of INGs in apoptosis.en_US
dc.identifier.citationMobahat, M. (2015). Regulation of Cell Death by The Drosophila ING Proteins (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27584en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27584
dc.identifier.urihttp://hdl.handle.net/11023/2234
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectGenetics
dc.subjectOncology
dc.subject.classificationINGen_US
dc.subject.classificationdING2en_US
dc.subject.classificationp53en_US
dc.subject.classificationApoptosisen_US
dc.subject.classificationDrosophilaen_US
dc.subject.classificationTumor Suppressoren_US
dc.titleRegulation of Cell Death by The Drosophila ING Proteins
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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