Chronic uphill and downhill exercise protocols do not lead to sarcomerogenesis in mouse skeletal muscle

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dc.contributor.authorMorais, Gustavo Paroschi
dc.contributor.authorda Rocha, Alisson Luiz
dc.contributor.authorNeave, Louise M.
dc.contributor.authorde Araújo Lucas, Guilherme
dc.contributor.authorLeonard, Timothy R.
dc.contributor.authorCarvalho, Andrea
dc.contributor.authorSilva, Adelino S. R.
dc.contributor.authorHerzog, Walter
dc.date.accessioned2020-01-22T15:50:47Z
dc.date.available2020-01-22T15:50:47Z
dc.date.issued2019-11-05
dc.description.abstractIt has been suggested that eccentric contraction (EC) is associated with increases in serially-arranged sarcomeres (sarcomerogenesis), while concentric contraction (CC) has been associated with serial sarcomeres decrease. Sarcomerogenesis following EC is thought to be a protective muscle adaptation, preventing muscle injury in future eccentric exercise bouts (repeated bout effect). However, the mechanisms underlying sarcomerogenesis in EC remain unknown, and the sarcomerogenic responses observed in response to EC and CC are contradictory. We measured sarcomere length, sarcomere length uniformity, serial sarcomere number, and fascicle length in gastrocnemius medialis, tibialis anterior, vastus medialis and vastus lateralis in sedentary (SED) mice, and in mice following protocols of moderate uphill (TRU) and downhill (TRD) training and uphill (OTU) and downhill (OTD) overtraining. We found pain sensitivity after the first bout of EC exercise on TRD and OTD followed by a normalized sensory response after four weeks of training, indicating a repeated bout effect. However, these findings were not associated with sarcomerogenesis, as serial sarcomere numbers did not increase in TRD and OTD skeletal muscle samples compared to controls (SED). However, we found a decrease in serial sarcomere number in VL and TA in OTU group mice, which was associated with a decrease in fascicle length and no change of sarcomere length at the tested joint configuration. We conclude that excessive concentric muscle contraction (OTU group mice), leads to a decrease in serial sarcomere number, while moderate or excessive eccentric training, did not result in sarcomerogenesis, as reported in the literature.en_US
dc.description.grantingagencyNatural Sciences and Engineering Research Council (NSERC)en_US
dc.identifier.citationMorais, G. P., da Rocha, A. L., Neave, L. M., de Araújo Lucas, G., Leonard, T. R., Carvalho, A., & Herzog, W. (2019). Chronic uphill and downhill exercise protocols do not lead to sarcomerogenesis in mouse skeletal muscle. http://dx.doi.org/10.1016/j.jbiomech.2019.109469en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jbiomech.2019.109469en_US
dc.identifier.urihttp://hdl.handle.net/1880/111538
dc.identifier.urihttps://doi.org/10.11575/PRISM/43910
dc.publisherJournal of Biomechanicsen_US
dc.publisher.departmentHuman Performance Laben_US
dc.publisher.facultyKinesiologyen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.institutionUniversity of São Pauloen_US
dc.publisher.institutionUniversity of British Columbiaen_US
dc.rightsUnless otherwise indicated, this material is protected by copyright and has been made available with authorization from the copyright owner. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0en_US
dc.subjectSarcomerogenesisEccentric contractionConcentric contractionOvertrainingRepeated bout effectMuscle sorenessen_US
dc.titleChronic uphill and downhill exercise protocols do not lead to sarcomerogenesis in mouse skeletal muscleen_US
dc.typejournal articleen_US
dc.typeacceptedVersionen_US
dc.typejournal articleen_US
dc.typeacceptedVersionen_US
ucalgary.item.requestcopyfalseen_US
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