Bioprocessing of human bone marrow mesecnchymal stem cells for the treatment of intervertebral disc degeneration
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2012
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Abstract
Lower back pain is a major medical problem in North America. From the biological point of view, the degeneration of intervertebral disc may be the main cause for lower back pain. The current study focused on using stem cell to differentiation into large number of healthy intervertebral disc cells. Human bone marrow mesenchymal stem cells (hBMMSCs) were used. This project has been divided into two parts: I. hBM-MSCs expansion, 2. hBM-MSCs differentiation. Experiments were carried out manipulating culture medium components ( oxygen tension, calcium, serum, pH) and agitation to improve the expansion of hBM-MSCs in bioreactors. After 33 days of culture under the developed protocol, approximately I 03 fold increase over the inoculation density had been obtained in suspension bioreactor culture. The differentiation of MSCs to nucleus pulposus-like cells was performed by using multiple growth factor cocktails and notochordal conditioned medium. There was higher expression of genes and proteins specific for nucleus pulposus cells, after exposure of MSCs to conditioned medium over 21 days than in basal medium.
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Bibliography: p. 154-168
Some pages are in colour.
Some pages are in colour.
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Citation
Yuan, Y. (2012). Bioprocessing of human bone marrow mesecnchymal stem cells for the treatment of intervertebral disc degeneration (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4814