Phenotypic Association between the Outer Membrane Protein OmpW and the Small Multi-Drug Resistance Protein EmrE in Escherichia coli

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atmire.migration.oldid2431
dc.contributor.advisorTurner, Raymond
dc.contributor.authorBeketskaia, Maria
dc.date.accessioned2014-09-02T14:53:52Z
dc.date.available2014-11-17T08:00:43Z
dc.date.issued2014-09-02
dc.date.submitted2014en
dc.description.abstractIn Escherichia coli, the small multidrug resistance (SMR) transporter protein EmrE confers host resistance to a broad range of toxic quaternary cationic compounds (QCCs) via proton motive force in the cytoplasmic membrane. Biologically produced QCCs also act as EmrE osmoprotectant substrates within the cell and participate in host pH regulation and osmotic tolerance. Although E. coli EmrE is one of the most well-characterized SMR members, it is unclear how the substrates that it transports into the periplasmic space escape across the outer membrane (OM) in Gram-negative bacteria. We tested the hypothesis that E. coli EmrE relies on an unidentified OM protein (OMP) to complete the extracellular release of its QCC. By conducting pH-based phenotypic growth screens, complementation and methyl viologen (MV) resistance assays, we have confirmed that EmrE relies on the presence of an OMP, specifically OmpW, to complete its extracellular substrate efflux across the OM.en_US
dc.identifier.citationBeketskaia, M. (2014). Phenotypic Association between the Outer Membrane Protein OmpW and the Small Multi-Drug Resistance Protein EmrE in Escherichia coli (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27333en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27333
dc.identifier.urihttp://hdl.handle.net/11023/1712
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subject.classificationEmrEen_US
dc.subject.classificationOmpWen_US
dc.subject.classificationSMRen_US
dc.subject.classificationMembraneen_US
dc.subject.classificationproteinen_US
dc.subject.classificationResistanceen_US
dc.subject.classificationporinen_US
dc.titlePhenotypic Association between the Outer Membrane Protein OmpW and the Small Multi-Drug Resistance Protein EmrE in Escherichia coli
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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