'High-sensitivity' Cardiac Troponin-T Assay Use in Horses: Analytical and Biological Validation, Post-Race Kinetics and Sampling Guidelines
Abstract
’High-sensitivity’ cardiac troponin assays are now the standard in human cardiology, but validation and exercise-kinetics in horses have not been investigated.
Objectives: Appropriately validate the hscTnT assay for use in horses, establish reference intervals, determine biological variation, explore race-induced hscTnT release kinetics, provide clinical sampling guidelines, and create model for sub-clinical cardiomyopathy. Methods/Results: Analytical performance of the assay in horses is verified. Reference intervals: upper 95th and 99th percentile of the hscTnT population distribution were 6.8 and 16.2ng/L in Non-Competition Horses, and 14.0 and 23.2ng/L in Racing-Thoroughbreds. Biological variation not appreciated due to number of horses below assay detection level. Racing-exercise caused peak hscTnT levels at 2-6h post-race, may approach 99th percentile URL, but declined by 12-24h. Clinician guidelines: normal horses should have declining levels by 12-24h and single values during this time-period should be <99th percentile URL:23.2ng/L. Occult sodium monensin cardiomyopathy model induced hscTnT elevations and ultrastructural cardiac changes.
Description
Keywords
Veterinary Science
Citation
Shields, E. (2016). 'High-sensitivity' Cardiac Troponin-T Assay Use in Horses: Analytical and Biological Validation, Post-Race Kinetics and Sampling Guidelines (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26367