Genome rearrangements in salmonella enterica serovar typhi

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dc.contributor.advisorSanderson, Kenneth E.
dc.contributor.authorKothapalli, Sushma
dc.date.accessioned2005-08-16T17:06:59Z
dc.date.available2005-08-16T17:06:59Z
dc.date.issued2004
dc.descriptionBibliography: p. 190-206en
dc.descriptionSome pages are in colour.en
dc.description.abstractThe order of core genes on the chromosomes of Escherichia coli K12 and of Salmonella enterica serovar Typhimurium LT2 is strongly conserved. However, the chromosome of Salmonella enterica serovar Typhi is rearranged due to homologous recombination between the rrn operons. PCR analysis detected the exact order and orientation of the seven I-Ceul fragments between operons in 136 independent wild-type strains of S. Typhi. Rearrangements within cultures of S. Typhi are common but cells with rearrangements are lost from culture. However, in spite of many rearrangements in S. Typhi, the PCR data support both the genome balance and gene dosage hypotheses for genome conservation. SARB63, a wild-type S. Typhi strain, has an unbalanced genome and a reduced growth rate in LB medium, whereas the revertents produced from SARB63 are rearranged back towards genomic balance and also have improved growth rates. Comparison of the sequenced strains of enteric bacteria for the terminus region suggests Dif as an important site in the terminus region.en
dc.format.extentxxi, 206 leaves : ill. ; 30 cm.en
dc.identifier.citationKothapalli, S. (2004). Genome rearrangements in salmonella enterica serovar typhi (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/17473en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/17473
dc.identifier.isbn0612975959en
dc.identifier.lccAC1 .T484 2004 K68en
dc.identifier.urihttp://hdl.handle.net/1880/41681
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleGenome rearrangements in salmonella enterica serovar typhi
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1512 520492029
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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