Characterization of Proteoglycan 4 Supramolecular Structure and its Effect on Lubricating Function

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dc.contributor.advisorSchmidt, Tannin
dc.contributor.advisorMacCallum, Justin
dc.contributor.authorMartens, Kayla
dc.contributor.committeememberDerksen, Darren
dc.contributor.committeememberSen, Arindom
dc.date2018-11
dc.date.accessioned2018-10-09T17:03:32Z
dc.date.available2018-10-09T17:03:32Z
dc.date.issued2018-09-14
dc.description.abstractProteoglycan 4 (PRG4) is a mucin-like glycoprotein that is a key synovial fluid constituent, functioning as a cartilage boundary lubricant. PRG4 has been suggested to exist as a supramolecular structure. The objectives of this thesis were to (1) characterize the supramolecular structure of recombinant human PRG4 (rhPRG4) and its dependency on concentration, (2) elucidate the molecular forces underlying the formation of the supramolecular structure, and (3) assess the lubricating ability of the supramolecular structure. rhPRG4’s ability to form a supramolecular structure and reduce friction was concentration dependent. Arginine (0.2M) disrupted the formation of the supramolecular structure of rhPRG4 and diminished its lubricating ability. Conversely, 20 mM calcium enhanced the formation of rhPRG4 supramolecular structure and enhanced its lubricating ability. These results suggest non-covalent interactions mediate rhPRG4’s supramolecular structure, shown here to exist for the first time, which in turn is a functionally (lubrication) determinant property.en_US
dc.identifier.citationMartens, K. (2018) Characterization of Proteoglycan 4 Supramolecular Structure and its Effect on Lubricating Function (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/33134en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/33134
dc.identifier.urihttp://hdl.handle.net/1880/108782
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultySchulich School of Engineering
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectChemistry
dc.subject.classificationEngineeringen_US
dc.subject.classificationEngineering--Biomedicalen_US
dc.titleCharacterization of Proteoglycan 4 Supramolecular Structure and its Effect on Lubricating Function
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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