Transductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities

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dc.contributor.authorKleiner, Manuel
dc.contributor.authorBushnell, Brian
dc.contributor.authorSanderson, Kenneth E
dc.contributor.authorHooper, Lora V
dc.contributor.authorDuerkop, Breck A
dc.date.accessioned2020-11-22T01:04:43Z
dc.date.available2020-11-22T01:04:43Z
dc.date.issued2020-11-15
dc.date.updated2020-11-22T01:04:43Z
dc.description.abstractAbstract Background Horizontal gene transfer (HGT) plays a central role in microbial evolution. Our understanding of the mechanisms, frequency, and taxonomic range of HGT in polymicrobial environments is limited, as we currently rely on historical HGT events inferred from genome sequencing and studies involving cultured microorganisms. We lack approaches to observe ongoing HGT in microbial communities. Results To address this knowledge gap, we developed a DNA sequencing-based “transductomics” approach that detects and characterizes microbial DNA transferred via transduction. We validated our approach using model systems representing a range of transduction modes and show that we can detect numerous classes of transducing DNA. Additionally, we show that we can use this methodology to obtain insights into DNA transduction among all major taxonomic groups of the intestinal microbiome. Conclusions The transductomics approach that we present here allows for the detection and characterization of genes that are potentially transferred between microbes in complex microbial communities at the time of measurement and thus provides insights into real-time ongoing horizontal gene transfer. This work extends the genomic toolkit for the broader study of mobile DNA within microbial communities and could be used to understand how phenotypes spread within microbiomes. Video Abstract
dc.identifier.citationMicrobiome. 2020 Nov 15;8(1):158
dc.identifier.doihttps://doi.org/10.1186/s40168-020-00935-5
dc.identifier.urihttp://hdl.handle.net/1880/112778
dc.identifier.urihttps://doi.org/10.11575/PRISM/45575
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.titleTransductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities
dc.typeJournal Article
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