Tid1 modulation of p53 ubiquitination in breast cancer cells

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dc.contributor.advisorKim, Sung-Woo
dc.contributor.authorKim, Shin Hwan
dc.date.accessioned2017-12-18T22:35:18Z
dc.date.available2017-12-18T22:35:18Z
dc.date.issued2012
dc.descriptionBibliography: p. 89-103en
dc.descriptionA page is in colour.en
dc.description.abstractIn this study, we showed that Tumorous Imaginal Disc 1 (Tidl) has the ability to indirectly modulate p53 ubiquitination in breast cancer cells. The over-expression of Tidl expression in MCF-7 cells led to suppression of p53 ubiquitination whereas its suppression led to enhanced ubiquitination. Tid 1 mediated suppression of p53 ubiquitination can enhance p53 half-life but not endogenous p53 level or p53 downstream targets. Over-expression of Tidl alone was unable to decrease the cell viability of MCF-7 cells but its combination with the proteasome inhibitor ALLN, there was a decrease in cell viability. A reduction in ATP level by Tidl over-expression is suggested to be one of the possible mechanisms that may suppress p53 ubiqutination. In summary, we propose that Tid 1 depletes cellular ATP levels, which in tum, inhibits ubiquitination­proteasome machinery, resulting in enhanced stability of p53.
dc.format.extentxiii, 103 leaves : ill. ; 30 cm.en
dc.identifier.citationKim, S. H. (2012). Tid1 modulation of p53 ubiquitination in breast cancer cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4942en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4942
dc.identifier.urihttp://hdl.handle.net/1880/105943
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleTid1 modulation of p53 ubiquitination in breast cancer cells
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2111 627942981
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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