Small-scale bioprocessing embryonic stem cells

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dc.contributor.advisorKallos, Michael S.
dc.contributor.advisorGates, Ian D.
dc.contributor.authorVan Winkle, Allison Patricia
dc.date.accessioned2017-12-18T22:20:08Z
dc.date.available2017-12-18T22:20:08Z
dc.date.issued2011
dc.descriptionBibliography: p. 121-139en
dc.descriptionSome pages are in colour.en
dc.description.abstractEmbryonic stem cells are capable of self-renewal and can be driven to differentiate into cell types from all three germ layers making them a source of cells with tremendous potential in the treatment of debilitating diseases and injuries. Expanding and differentiating cells in suspension bioreactors is a critical step towards widespread use of stem cells in medicine. The goal of this project was to develop a small-scale microbioreactor array for high throughput experimentation in order to improve the protocols used for expansion and differentiation of populations of embryonic stem cells. Initial experiments were performed with a small prototype array with the aim of replicating the cell expansion obtained in standard 100 mL bioreactors. These results of these experiments led to the hypothesis that the fluid environment created in the bioreactors was substantially different between these two scales. A finite element simulation was created to compare the hydrodynamic environments. Following this, a numerical simulation was used to study the effect of diffusion on embryonic stem cell differentiation in aggregate form. These simulation studies have important implications for future bioprocess design.
dc.format.extentxvii, 142 leaves : ill. ; 30 cm.en
dc.identifier.citationVan Winkle, A. P. (2011). Small-scale bioprocessing embryonic stem cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4176en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4176
dc.identifier.urihttp://hdl.handle.net/1880/105177
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleSmall-scale bioprocessing embryonic stem cells
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2051 627942893
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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