Novel Analytical Method for Trace Level Quantification of Disinfection By-Products in Recycled Wastewaters

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dc.contributor.advisorKimura, Susana Y.
dc.contributor.authorOrtega, Alejandro Rene
dc.contributor.committeememberThurbide, Kevin B.
dc.contributor.committeememberAchari, Gopal
dc.contributor.committeemembervan Humbeck, Jeffrey F.
dc.date2021-02
dc.date.accessioned2020-12-10T19:13:38Z
dc.date.available2020-12-10T19:13:38Z
dc.date.issued2020-12-08
dc.description.abstractThe work of this thesis quantifies and characterizes unregulated priority disinfection by-products (DBPs) in a full-scale potable wastewater reuse treatment plant. DBPs are small organic molecules formed from the reaction between natural organic matter (NOM) and disinfectants. Unregulated DBPs display genotoxic, cytotoxic and potentially carcinogenic properties to humans. Wastewater reuse is an area of growing interest as freshwater sources are being depleted due to increasing human population and climate change. Secondary effluent treated with microfiltration (UF), ozone, and reverse osmosis waters were disinfected to observe the change in DBP composition throughout various stages of the treatment train process. First, a novel multiple reaction monitoring (MRM) method was developed on a gas chromatography – triple quadrupole mass spectrometer (GC-MS/MS) that quantifies 25 DBPs formed from chlorinated and chloraminated wastewater effluents. The order of optimization of each DBP class involved the determination of chemical transitions, collision energies, dwell times, time segments and determination of method detection limits. The optimization of these parameters led to a highly sensitive quantification method for the DBPs found in this method. Method detection limits ranged from 2.0 - 68.9 ng/L. Next, a liquid – liquid extraction (LLE) method for sample analysis was modified to account for the large increase in sensitivity that comes with a triple quadrupole. MS/MS instruments are highly advantageous because of the selection of specific chemical transitions for quantification. This specificity results in precise quantification due to the large signal to noise ratio of each chemical fragment at trace levels. The LLE method was reduced 10 fold in terms of time, reagents, and sample volume compared to published methods. The last part of this work looked at the DBP composition of each water matrix across three seasons of the year. Specifically, waters were sampled in fall, winter, and summer to observe how the change in precursors affected DBP formation.en_US
dc.identifier.citationOrtega, A. R. (2020). Novel Analytical Method for Trace Level Quantification of Disinfection By-Products in Recycled Wastewaters (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/38424
dc.identifier.urihttp://hdl.handle.net/1880/112816
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectDisinfection By-productsen_US
dc.subjectDBPsen_US
dc.subjectWastewater Reuseen_US
dc.subjectAnalytical Chemistryen_US
dc.subject.classificationChemistry--Analyticalen_US
dc.subject.classificationEngineering--Chemicalen_US
dc.subject.classificationEngineering--Environmentalen_US
dc.titleNovel Analytical Method for Trace Level Quantification of Disinfection By-Products in Recycled Wastewatersen_US
dc.typemaster thesisen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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