Intermediate conductance calcium-activated potassium channels modulate summation of parallel fiber input in cerebellar Purkinje cells

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dc.contributor.authorEngbers, Jordan D .T.
dc.contributor.authorAnderson, Dustin M.
dc.contributor.authorAsmara, Hadhimulya
dc.contributor.authorRehak, Renata
dc.contributor.authorMehaffey, W. Hamish
dc.contributor.authorHameed, Shahid
dc.contributor.authorMcKay, Bruce E.
dc.contributor.authorKruskic, Mirna
dc.contributor.authorZamponi, Gerald W.
dc.contributor.authorTurner, Ray W.
dc.date.accessioned2018-05-22T17:26:48Z
dc.date.available2018-05-22T17:26:48Z
dc.date.issued2012-02-14
dc.description.abstractEncoding sensory input requires the expression of postsynaptic ion channels to transform key features of afferent input to an appropriate pattern of spike output. Although Ca(2+)-activated K(+) channels are known to control spike frequency in central neurons, Ca(2+)-activated K(+) channels of intermediate conductance (KCa3.1) are believed to be restricted to peripheral neurons. We now report that cerebellar Purkinje cells express KCa3.1 channels, as evidenced through single-cell RT-PCR, immunocytochemistry, pharmacology, and single-channel recordings. Furthermore, KCa3.1 channels coimmunoprecipitate and interact with low voltage-activated Cav3.2 Ca(2+) channels at the nanodomain level to support a previously undescribed transient voltage- and Ca(2+)-dependent current. As a result, subthreshold parallel fiber excitatory postsynaptic potentials (EPSPs) activate Cav3 Ca(2+) influx to trigger a KCa3.1-mediated regulation of the EPSP and subsequent after-hyperpolarization. The Cav3-KCa3.1 complex provides powerful control over temporal summation of EPSPs, effectively suppressing low frequencies of parallel fiber input. KCa3.1 channels thus contribute to a high-pass filter that allows Purkinje cells to respond preferentially to high-frequency parallel fiber bursts characteristic of sensory input.en_US
dc.identifier.citationEngbers, J. D. T., Anderson, D., Asmara, H., Rehak, R., Mehaffey, W. H., Hameed, S., … Turner, R. W. (2012). Intermediate conductance calcium-activated potassium channels modulate summation of parallel fiber input in cerebellar Purkinje cells. Proceedings of the National Academy of Sciences, 109(7), 2601–2606. https://doi.org/10.1073/pnas.1115024109en_US
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1115024109en_US
dc.identifier.urihttp://hdl.handle.net/1880/106649
dc.identifier.urihttps://doi.org/10.11575/PRISM/43842
dc.language.isoenen_US
dc.publisherProceedings of the National Academy of Sciencesen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleIntermediate conductance calcium-activated potassium channels modulate summation of parallel fiber input in cerebellar Purkinje cellsen_US
dc.typejournal article
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