Effects of transportation to and commingling at an auction market on the bacterial communities of the respiratory tract of beef cattle

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dc.contributor.advisorTimsit, Édouard
dc.contributor.advisorAlexander, Trevor W.
dc.contributor.authorStroebel, Christina Maria
dc.contributor.committeememberDe Buck, Jeroen M.
dc.contributor.committeememberJanzen, Eugene D.
dc.date2018-11
dc.date.accessioned2018-07-26T14:01:18Z
dc.date.available2018-07-26T14:01:18Z
dc.date.issued2018-07-12
dc.description.abstractCommingling at auction markets is considered a major predisposing factor for bacterial bronchopneumonia (BP) in beef cattle. However, the effects of commingling on the respiratory tract bacterial communities is largely unknown. Therefore, the objective of the thesis was to study the effects of transportation to and commingling at an auction market on the nasopharyngeal and tracheal bacterial communities of recently weaned calves using culture-dependent and independent methods. Two groups of 30 Angus-cross heifers were studied from weaning at the ranches of origin to 28 d after arrival at a feedlot. For each group, half the heifers were either transported directly to a feedlot after weaning (RANC) or transported to and commingled at an auction market for 24 hrs before being placed in a feedlot (AUCT). Heifers from both groups received vaccines against major respiratory viruses and a parenteral injection of a long acting macrolide (tildipirosin) at on-arrival processing (d2). Deep nasal swabs (DNS) and trans-tracheal aspirates (TTA) were collected at weaning (d0) and at on-arrival processing at the feedlot (d2). Deep nasal swab sampling only was then repeated 7 days (d9) and 28 days (d30) after arrival. Bacterial culture and 16S rRNA sequencing did not reveal difference in the nasopharyngeal and tracheal bacterial communities between RANC and AUCT at any sampling days. However, both time after arrival and feedlot where calves were placed affected diversity and composition of the nasopharyngeal bacterial communities. In both groups, there was a reduction in bacterial diversity and a large increase in Mycoplasma after feedlot placement, especially seven days after on-arrival processing. Furthermore, we observed the horizontal transmission of a multi-resistant strain of Pasteurella multocida among calves at d9 and d30 in one of the two groups of heifers. Based on these findings, we concluded that transportation to and commingling at an auction market for 24 hrs did not significantly influence the composition and diversity of the nasopharyngeal and tracheal bacterial communities of recently weaned calves.en_US
dc.identifier.citationStroebel, C. M. (2018). Effects of transportation to and commingling at an auction market on the bacterial communities of the respiratory tract of beef cattle (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32694en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/32694
dc.identifier.urihttp://hdl.handle.net/1880/107513
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBovine Respiratory Disease
dc.subjectPathogen transmission
dc.subjectAuction Market
dc.subjectMicrobiota
dc.subjectMetagenomics
dc.subject16S rRNA sequencing
dc.subjectFeedlot
dc.subject.classificationVeterinary Scienceen_US
dc.titleEffects of transportation to and commingling at an auction market on the bacterial communities of the respiratory tract of beef cattle
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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