Assessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purification

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dc.contributor.authorRohatensky, Mitchell G
dc.contributor.authorLivingstone, Devon M
dc.contributor.authorMintchev, Paul
dc.contributor.authorBarnes, Heather K
dc.contributor.authorNakoneshny, Steven C
dc.contributor.authorDemetrick, Douglas J
dc.contributor.authorDort, Joseph C
dc.contributor.authorvan Marle, Guido
dc.date.accessioned2018-02-13T22:21:56Z
dc.date.available2018-02-13T22:21:56Z
dc.date.issued2018-02-08
dc.descriptionCalgary Surgical Research Development Fund, the Ohlson Research Initiative, Alberta Innovates Health Solutionsen_US
dc.description.abstractOropharyngeal Squamous Cell Carcinoma (OPSCC) is increasing in incidence despite a decline in traditional risk factors. Human Papilloma Virus (HPV), specifically subtypes 16, 18, 31 and 35, has been implicated as the high-risk etiologic agent. HPV positive cancers have a significantly better prognosis than HPV negative cancers of comparable stage, and may benefit from different treatment regimens. Currently, HPV related carcinogenesis is established indirectly through Immunohistochemistry (IHC) staining for p16, a tumour suppressor gene, or polymerase chain reaction (PCR) that directly tests for HPV DNA in biopsied tissue. Loop mediated isothermal amplification (LAMP) is more accurate than IHC, more rapid than PCR and is significantly less costly. In previous work we showed that a subtype specific HPV LAMP assay performed similar to PCR on purified DNA. In this study we examined the performance of this LAMP assay without DNA purification.en_US
dc.description.grantingagencyOtheren_US
dc.identifier.citationRohatensky, M. G., Livingstone, D. M., Mintchev, P., Barnes, H. K., Nakoneshny, S. C., Demetrick, D. J., ... & van Marle, G. (2018). Assessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purification. BMC Cancer, 18(1), 166.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/s12885-018-4087-1en_US
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/1880/106394
dc.identifier.urihttps://doi.org/10.11575/PRISM/43847
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.publisher.departmentMicrobiology, Immunology & Infectious Diseasesen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.hasversionPublisher's versionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://www.biomedcentral.com/terms-and-conditionsen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.subjectLAMPen_US
dc.subjectHPVen
dc.subjectOPSCCen
dc.subjectLoop-mediated isothermal amplificationen
dc.subjectOropharynxen
dc.titleAssessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purificationen_US
dc.typejournal article
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