Microscale Tissue Engineering for the Study and Treatment of Diabetes

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dc.contributor.advisorUngrin, Mark D.
dc.contributor.authorYu, Yang
dc.contributor.committeememberHuang, Carol T. L.
dc.contributor.committeememberRancourt, Derrick E.
dc.date2019-06
dc.date.accessioned2018-12-13T19:43:10Z
dc.date.available2018-12-13T19:43:10Z
dc.date.issued2018-12-12
dc.description.abstractIslet transplantation is a promising approach to the treatment of insulin-dependent diabetes. However, a major clinical challenge is inefficient survival and engraftment of the transplanted material. This has been associated with insufficient oxygen and nutrition delivery after loss of the endogenous capillaries, and stress induced during islet isolation and culture. Quantitative modelling of oxygen delivery predicts significant advantages for smaller islets, and consistent with this concept, smaller human islets have also performed better than larger ones in clinical settings. In order to understand and overcome these limitations for both research and clinical applications, we have established a microscale tissue engineering approach that is capable of consistently and efficiently generating size-controlled pseudoislets from human donor islets, yielding improved survival and function both in vitro and in vivo. We then combined this platform with advanced statistical methodologies and laboratory automation systems to enable assessment of large numbers of modifying factors (and their interactions) identified from the literature in parallel. This project has yielded a substantial improvement in the consistency and efficacy of islet cell packaging for transplantation, and laid a foundation for rapid transition to the clinic.en_US
dc.identifier.citationYu, Y. (2018). Microscale Tissue Engineering for the Study and Treatment of Diabetes (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/34961
dc.identifier.urihttp://hdl.handle.net/1880/109339
dc.language.isoenen_US
dc.publisher.facultySchulich School of Engineeringen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectIsleten_US
dc.subjectTransplanten_US
dc.subjectPseudoisleten_US
dc.subjectMicro-tissue Engineeringen_US
dc.subjectDesign of Experimenten_US
dc.subjectProstanoidsen_US
dc.subject.classificationEngineering--Biomedicalen_US
dc.titleMicroscale Tissue Engineering for the Study and Treatment of Diabetesen_US
dc.typedoctoral thesisen_US
thesis.degree.disciplineEngineering – Biomedicalen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameDoctor of Philosophy (PhD)en_US
ucalgary.item.requestcopytrue
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