Capillary Gas Chromatographic Separation of Carboxylic Acids Using an Acidic Water Stationary Phase

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dc.contributor.authorThurbide, Kevin
dc.contributor.authorDarko, Ernest
dc.date.accessioned2017-07-27T17:10:21Z
dc.date.available2017-07-27T17:10:21Z
dc.date.issued2017-08-01
dc.description.abstractAn acidic water stationary phase is used for the analysis of carboxylic acids in capillary gas chromatography (GC). Under regular pH 7 operating conditions, these analytes are largely ionized and elute poorly, if at all, from the water phase. However, by adjusting the phase to pH 2, it is found that various acids are neutralized and can be readily eluted and separated in the system. Sulfamic acid is found to provide a stable pH for the water phase over time, whereas hydrochloric acid and other more volatile additives quickly evaporate from the column. Under optimal low pH conditions, the acidic analytes yield good peak shape and are readily observed for masses investigated down to 5 ng on-column. By comparison, on a conventional non-polar capillary GC column, the same analytes display threefold more peak tailing and are not detected for masses below 30 ng on-column. Through altering the phase pH, it is found that the selectivity between certain analytes can be potentially enhanced depending on their respective pKa values and/or ionizability. The analysis of various different samples containing carboxylic acids is demonstrated and the results indicate that this approach can possibly offer unique and beneficial selectivity in such determinations.en_US
dc.description.refereedYesen_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/30261
dc.identifier.urihttp://hdl.handle.net/1880/52155
dc.language.isoenen_US
dc.publisherThe final publication is available at Springer via http://dx.doi.org/10.1007/s10337-017-3333-zen_US
dc.publisher.departmentChemistryen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.subjectGas chromatgraphyen_US
dc.subjectAcidicen_US
dc.subjectWateren_US
dc.subjectStationary phaseen_US
dc.subjectCarboxylic acidsen_US
dc.titleCapillary Gas Chromatographic Separation of Carboxylic Acids Using an Acidic Water Stationary Phaseen_US
dc.typejournal article
thesis.degree.disciplineChemistryen_US
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