Csde1, an RNA Binding Protein, Modulates Neuronal Subtype Specification

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dc.contributor.advisorYang, Guang
dc.contributor.authorHarvey, Emily M.
dc.contributor.committeememberHuang, Peng
dc.contributor.committeememberKurrasch, Deborah
dc.dateFall Convocation
dc.date.accessioned2022-11-15T17:42:34Z
dc.date.embargolift2022-09-20
dc.date.issued2021-09-20
dc.description.abstractNeuronal diversity is the root of complex function in the cerebral cortex. During cortical development, neural stem cells give rise to neurons. Neurons are a diverse cell type and can be grouped into many subtypes, each with distinct functional identities. Neurogenesis and neuronal subtype specification are carefully regulated, both temporally and spatially, by programmed gene expression. One mechanism controlling gene expression is translational regulation, which alters levels of protein synthesis. Translational regulation orchestrated by RNA binding proteins allows dynamic alterations in protein expression. Here I determine that Cold-shock domain containing E1 (Csde1), an RNA binding protein, regulates the specification of neuronal subtypes in the cerebral cortex. In the developing murine cortex, Csde1 is highly expressed in newborn neurons of the cortical plate during development. I show that reduced Csde1 expression alters neuronal subtype in the embryonic and post-natal cortex. Csde1 reduction disrupts the distribution of neuronal subtypes within the cortical plate. Additionally, reduced Csde1 expression increases mixed neuronal subtype identity. Together, this indicates that the specification of neuronal subtypes is subject to translational regulation, and that Csde1 activity is essential for specification of neurons in the cortex.
dc.identifier.citationHarvey, E. M. (2021). Csde1, an RNA Binding Protein, Modulates Neuronal Subtype Specification (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.urihttp://hdl.handle.net/1880/115448
dc.identifier.urihttps://dx.doi.org/10.11575/PRISM/40415
dc.language.isoenen
dc.language.isoEnglish
dc.publisher.facultyGraduate Studiesen
dc.publisher.facultyCumming School of Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en
dc.subjectAutism
dc.subject.classificationBiology--Neuroscience
dc.subject.classificationBiology--Molecular
dc.subject.classificationBiology--Genetics
dc.subject.classificationHealth Sciences--Human Development
dc.titleCsde1, an RNA Binding Protein, Modulates Neuronal Subtype Specification
dc.typemaster thesis
thesis.degree.disciplineMedicine – Biochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgaryen
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
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