The Role of Extracellular Vesicles in Giardia Microbiota Interactions

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dc.contributor.advisorBuret, Andre
dc.contributor.authorSiddiq, Affan
dc.contributor.committeememberMcKay, Derek
dc.contributor.committeememberGedamu, Lashitew
dc.date2021-11
dc.date.accessioned2021-06-21T21:55:49Z
dc.date.available2021-06-21T21:55:49Z
dc.date.issued2021-06-16
dc.description.abstractGiardia duodenalis is one of the most common cause of parasitic driven diarrheal diseases worldwide with over 200 million cases. Limited evidence indicates that the parasite is capable of shedding extracellular vesicles (EVs) which have been implicated in pathogenesis. In this work, we characterized Giardia EVs using state-of-the-art methods such as nanoparticle track analysis, transmission electron microscopy, proteomics, and small RNA sequencing. The effects of Giardia-derived EVs on the growth and behavior of commensal bacteria were examined. Our results indicate that Giardia produces EVs with characteristic morphology and these EVs contained virulence factors, and a variety of small RNA molecules including miRNAs. The EVs were shown to affect the growth and motility of commensal bacteria by exhibiting bacteriostatic effects, increasing bacterial motility, promoting bacterial adhesion to epithelial cells and decreasing their ability to form biofilms. The effects of EVs on bacterial motility, adhesion and biofilm forming ability were found to be, in part, mediated by the RNA content of EVs. Taken together, these findings highlight the novel role of Giardia derived EVs in mediating trans-kingdom crosstalk with the commensal bacteria and contribute to our understanding of EVs in Giardia pathogenesis.en_US
dc.identifier.citationSiddiq, A. (2021). The Role of Extracellular Vesicles in Giardia Microbiota Interactions (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/38943
dc.identifier.urihttp://hdl.handle.net/1880/113521
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectGiardiaen_US
dc.subjectExtracellular vesiclesen_US
dc.subjectMicrobiotaen_US
dc.subjectHost-pathogen interactionsen_US
dc.subjectDysbiosisen_US
dc.subject.classificationMicrobiologyen_US
dc.titleThe Role of Extracellular Vesicles in Giardia Microbiota Interactionsen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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