Interactions of Rabconnectin-3 with Cav2 calcium channels
| dc.contributor.author | Gandini, Maria A | |
| dc.contributor.author | Souza, Ivana A | |
| dc.contributor.author | Fan, Jing | |
| dc.contributor.author | Li, Katherine | |
| dc.contributor.author | Wang, Decheng | |
| dc.contributor.author | Zamponi, Gerald W | |
| dc.date.accessioned | 2019-06-30T00:15:43Z | |
| dc.date.available | 2019-06-30T00:15:43Z | |
| dc.date.issued | 2019-06-28 | |
| dc.date.updated | 2019-06-30T00:15:43Z | |
| dc.description.abstract | Abstract This study describes the interaction between Cav2 calcium channels and Rabconnectin-3, a di-subunit protein that is associated with synaptic vesicles. Immunostaining reveals that both Rabconnectin-3α (RB-3α) and Rabconnectin-3β (RB-3β) are colocalized in mouse hippocampal neurons. Co-immunoprecipitations from brain tissue is consistent with the formation of a protein complex between RB-3α and RB-3β and both Cav2.2 and the related Cav2.1 calcium channel. The coexpression of either RB-3α or RB-3β with Cav2.2 calcium channels in tsA-201 cells led to a reduction in Cav2.2 current density without any effects on the voltage-dependence of activation or inactivation. Coexpression of both Rabconnectin-3 subunits did not cause an additive effect on current densities. Finally, the presence of Rabconnectin-3 did not interfere with μ-opioid receptor mediated Gβγ modulation of Cav2.2 channels. Altogether, our findings show that Rabconnectin-3 has the propensity to regulate calcium entry mediated by Cav2.2 channels. | |
| dc.identifier.citation | Molecular Brain. 2019 Jun 28;12(1):62 | |
| dc.identifier.doi | https://doi.org/10.1186/s13041-019-0483-y | |
| dc.identifier.uri | http://hdl.handle.net/1880/110541 | |
| dc.identifier.uri | https://doi.org/10.11575/PRISM/45995 | |
| dc.language.rfc3066 | en | |
| dc.rights.holder | The Author(s). | |
| dc.title | Interactions of Rabconnectin-3 with Cav2 calcium channels | |
| dc.type | Journal Article |