Monitoring angiogenesis using a human compatible calibration for broadband near-infrared spectroscopy
Date
2013-01
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Photo-optical Instrumentation Engineers (SPIE)
Abstract
Angiogenesis is a hallmark of many conditions, including cancer, stroke, vascular disease, diabetes, and
high-altitude exposure. We have previously shown that one can study angiogenesis in animal models by using total
hemoglobin (tHb) as a marker of cerebral blood volume (CBV), measured using broadband near-infrared spectroscopy
(bNIRS). However, the method was not suitable for patients as global anoxia was used for the calibration. Here
we determine if angiogenesis could be detected using a calibration method that could be applied to patients. CBV,
as a marker of angiogenesis, is quantified in a rat cortex before and after hypoxia acclimation. Rats are acclimated at
370-mmHg pressure for three weeks, while rats in the control group are housed under the same conditions, but
under normal pressure. CBV increased in each animal in the acclimation group. The mean CBV (%volumeāvolume)
is 3.49% 0.43% (mean SD) before acclimation for the experimental group, and 4.76% 0.29% after acclimation.
The CBV for the control group is 3.28% 0.75%, and 3.09% 0.48% for the two measurements.
This demonstrates that angiogenesis can be monitored noninvasively over time using a bNIRS system with a calibration
method that is compatible with human use and less stressful for studies using animals.
Description
Keywords
near-infrared spectroscopy, near infrared, hemoglobin, acclimation, cerebral blood volume, angiogenesis, hypoxia, brain
Citation
Yang R. et. al. (2013). Monitoring angiogenesis using a human compatible calibration for broadband near-infrared spectroscopy. Journal of Biomedical Optics. 18(1): 016011