Prototype design and temperature uniformity on loop-mediated isothermal amplification temperature control system
Date
2012
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Abstract
Malaria diagnostics needs to be rapid, accurate and cost effective to ensure proper treatment is received. Loop-mediated isothermal amplification (LAMP) DNA amplification method has these characteristics. This nucleic acid amplification test (NAT) can identify parasite genus and species accurately and even detect drug resistance. Compared to conventional microscopy, which is the main tool used for detection and identification in the field, the LAMP method has significantly higher sensitivity and specificity. However, the problem with LAMP is the cost of the reagents, and the cost and operation of the equipment to run (heating blocks/thermal cyders) and reliably read out the assays (spectrophotometers).
This dissertation discusses about low-cost prototype design of heating block and temperature uniformity control. For the prototype design, Chapter IV introduces the constituents of hardware system first and then introduces the design of heating and cooling system based on Thermoelectric Cooler (TEC) and finally introduces two assistant departments-heated lid and fan briefly. For the research on heating block temperature uniformity, Chapter V includes establishing the finite element simulation model of the sample block to obtain the temperature field. From the result of the simulation, the exiting model already has good temperature uniformity. Further work would include testing the model experimentally. Finally, Chapter VI includes research on marketing analysis for our device in developing countries.
Description
Bibliography: p. 87-91
Many pages are in colour.
Many pages are in colour.
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Citation
Cao, R. (2012). Prototype design and temperature uniformity on loop-mediated isothermal amplification temperature control system (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4820