Prototype design and temperature uniformity on loop-mediated isothermal amplification temperature control system

Simple item page
dc.contributor.advisorKallos, Michael S.
dc.contributor.authorCao, Rui
dc.date.accessioned2017-12-18T22:32:34Z
dc.date.available2017-12-18T22:32:34Z
dc.date.issued2012
dc.descriptionBibliography: p. 87-91en
dc.descriptionMany pages are in colour.en
dc.description.abstractMalaria diagnostics needs to be rapid, accurate and cost effective to ensure proper treatment is received. Loop-mediated isothermal amplification (LAMP) DNA amplification method has these characteristics. This nucleic acid amplification test (NAT) can identify parasite genus and species accurately and even detect drug resistance. Compared to conventional microscopy, which is the main tool used for detection and identification in the field, the LAMP method has significantly higher sensitivity and specificity. However, the problem with LAMP is the cost of the reagents, and the cost and operation of the equipment to run (heating blocks/thermal cyders) and reliably read out the assays (spectrophotometers). This dissertation discusses about low-cost prototype design of heating block and temperature uniformity control. For the prototype design, Chapter IV introduces the constituents of hardware system first and then introduces the design of heating and cooling system based on Thermoelectric Cooler (TEC) and finally introduces two assistant departments-heated lid and fan briefly. For the research on heating block temperature uniformity, Chapter V includes establishing the finite element simulation model of the sample block to obtain the temperature field. From the result of the simulation, the exiting model already has good temperature uniformity. Further work would include testing the model experimentally. Finally, Chapter VI includes research on marketing analysis for our device in developing countries.
dc.format.extentxiv, 91 leaves : ill. ; 30 cm.en
dc.identifier.citationCao, R. (2012). Prototype design and temperature uniformity on loop-mediated isothermal amplification temperature control system (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4820en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4820
dc.identifier.urihttp://hdl.handle.net/1880/105821
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titlePrototype design and temperature uniformity on loop-mediated isothermal amplification temperature control system
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Engineering (MEng)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2083 627942955
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_Cao_2012.pdf
Size:
41.14 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
Collections
Legacy Theses